Détails de la publication

Molecular characterization and Antibiotic resistance profiles of Escherichia coli extended-spectrum β-lactamases producer strains isolated from urine samples in Benin

Détails de la publication

• Type:Article
• Domaine: Santé humaine
• Année de publication: 2018
• Auteurs: MOUSSE W., SINA H., WELE M., CHABI N., DAH-NOUVLESSOUNON D., BADE F. T., CHABI F., ANAGONOU S., ADJANOHOUN A., BABA-MOUSSA L.,
• Cultures: Urinary Infections
• Couverture géographique: Bénin
• Mots clés: Urinary Infections; Antibiotic; Resistance; ESBL; Benin

Résumé de la publication

Urinary tract infections are the second common reason of medical consultations and antibiotics prescription. Escherichia coli is known to cause most urinary tract infections. The aim of this study was to characterize and determine the antibiotic resistance profile of E. coli extended-spectrum β- lactamases (ESBL) producer strains isolated from urine samples. The urine samples collected came from hospitalized and non-hospitalized patient European Scientific Journal October 2018 edition Vol.14, No.30 ISSN: 1857 – 7881 (Print) e - ISSN 1857- 7431 324 referred to Hubert Koutoukou Manga (HKM), National and University Hospital Center (Cotonou, Benin). The resistance to antibiotics was determined according to the disk diffusion method. The production of penicillinase and ESBLs was researched respectively by the acidimetric test and double disk synergy method. The presences of genes encoding β- lactamases were detected by Polymerase Chain Reaction (PCR). Our data revealed that 60 % of E. coli strains (101) were isolated from female patients. Also, 69.31%of the strains were isolated from non-hospitalized patients. The high resistance levels were recorded with amoxicillin (96.04 %) and amoxicillin + clavulanic acid (66.34 %). Twenty percent (20%) of strains were ESBLs. Among ESBLs strains, 70% comes from non-hospitalized patients. Eighty percent of E. coli strains produced penicillinase among which 25 % were ESBL producers. All the ESBL producers strains carried blaTEM gene whereas only 30 % carried the blaSHV gene. This study updates the data on the prevalence to antibiotic resistance of E. coli ESBL producers strains for better management of urinary tract infections.